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e clip style pin multi blot replicator  (AutoMate Scientific Inc)


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    Structured Review

    AutoMate Scientific Inc e clip style pin multi blot replicator
    Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin <t>replicator</t> and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.
    E Clip Style Pin Multi Blot Replicator, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 94/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e clip style pin multi blot replicator/product/AutoMate Scientific Inc
    Average 94 stars, based on 129 article reviews
    e clip style pin multi blot replicator - by Bioz Stars, 2026-04
    94/100 stars

    Images

    1) Product Images from "A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae"

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2021.100868

    Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.
    Figure Legend Snippet: Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Techniques Used: Mutagenesis, Expressing, Flow Cytometry


    Figure Legend Snippet:

    Techniques Used: Recombinant, Clone Assay, Software, Microscopy



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    e clip style pin multi blot replicator  (AutoMate Scientific Inc)
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    AutoMate Scientific Inc e clip style pin multi blot replicator
    Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin <t>replicator</t> and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.
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    Image Search Results


    Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet: Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Article Snippet: Sterilizing the pin tools Note: All pinning steps are performed using a 96 Floating E-Clip style Pin Multi-Blot Replicator (V&P Scientific).

    Techniques: Mutagenesis, Expressing, Flow Cytometry

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet:

    Article Snippet: Sterilizing the pin tools Note: All pinning steps are performed using a 96 Floating E-Clip style Pin Multi-Blot Replicator (V&P Scientific).

    Techniques: Recombinant, Clone Assay, Software, Microscopy

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet:

    Article Snippet: 96 Floating E-Clip style Pin Multi-Blot Replicator , V&P Scientific , VP 408FS2AS.

    Techniques: Recombinant, Clone Assay, Software, Microscopy